The story takes place in an ancient South American empire. The young king of this empire, Kuzco, was extremely vain. In order to celebrate his 18th birthday, he decided to build a palace on a mountain near the capital of the empire. The residents who had lived on the mountain for generations were in trouble for a while. The villager Pacha came to the palace and asked Kuzco to consider the situation of the local residents and withdraw his order, but was rejected by Kuzco. At the same time, the evil priestess Yzma was ready to poison and kill Kuzco to seize the throne, but her follower Kronk made a mistake with the poison and mistakenly gave the transformation potion to Kuzco. So, Kuzco turned into a mule and got on Pacha's car by mistake to go to the village on the mountain. After Yzma learned about it, he launched a series of pursuits against Kuzco, and Kuzco also launched a counterattack with the help of Pacha and others!